rat anti mouse monoclonal f4 80  (Bio-Rad)

Journal: Frontiers in Immunology

Article Title: Limosilactobacillus reuteri alleviates proinflammatory T-cell-mediated liver injury and transcriptomic changes in immunocompromised mice

doi: 10.3389/fimmu.2026.1713120

Figure Lengend Snippet: Liver macrophage (MΦ) evaluation from RAG1KO mice after adoptive transfer of CD4 + T cells, comparing RAG1KOATSFC or RAG1KOATSFL with WT and RAG1KOnoAT groups. (A) Representatives of liver tissue sections from mice stained with F4/80 antibody from WT, RAG1KOnoAT, RAG1KOATSFC, and RAG1KOATSFL groups. F4/80-positive MΦs are indicated by dark brown staining. Scale bar = 50 µm with × 200 magnification. (B) Quantitation of the number of F4/80-positive cells per high-power field (HPF) in the liver (counted under × 400 magnification), comparing the different groups ( n = 4–10 mice per group). (C) Liver genes that were upregulated by SF-CD4 + T-cell transfer and downregulated by probiotic-modulated SF-CD4 + T (Prob-SF-CD4 + T)-cell transfer. The diagram shows genes associated with MΦ polarization, categorized by their association with M1, M2, or both phenotypes. Colored shapes indicate gene associations with specific metabolic pathways: lipid (yellow), iron (light blue), bile acid (gray), glucose (green), and amino acid (light pink). Triangles represent genes in liver metabolism, and circles represent genes in MΦ metabolism ( n = 3 mice per group). (D) A heatmap displaying the expression of 36 genes associated with MΦ polarization and metabolism across the three study groups: RAG1KOnoAT, RAG1KOATSFC, and RAG1KOATSFL ( n = 3 mice per group).

Article Snippet: A rat monoclonal antimouse antibody F4/80 (MCA497R, Bio-Rad Laboratories, Hercules, CA, USA) was used for staining at a 1:200 dilution and incubated at 4°C overnight.

Techniques: Adoptive Transfer Assay, Staining, Quantitation Assay, Expressing

Journal: Cell reports

Article Title: Fibroblast-derived alarmin promotes oral wound healing by activating regulatory T cells that relay pro-angiogenic and anti-inflammatory responses

doi: 10.1016/j.celrep.2025.116829

Figure Lengend Snippet: (A) Feature plots for monocyte/macrophage gene markers such as Adgre1 , Itgam , Cd68 , and Tlr2 . (B) Left: UMAP highlighting M1 (orange) and M2 (red) subclusters. Right: feature plots of anti-inflammatory genes (top; Apoe , Mrc1 , Trem2 , and Il10 ) and pro-inflammatory genes (bottom; Tnf , Il1b , Il1a , and Cxcl2 ). (C) Top: violin plots of anti-inflammatory markers such as Il10 , Il1rn , Arg1 , Apoe , and Trem2 in the M2 cluster, comparing control versus ΔIL33 Col1α2 mice. Bottom: violin plots of pro-inflammatory markers such as Il1a , Cxcl2 , Tnfrsf1b , Ctsc , and Tnfaip3 in the M1 cluster. * p .adj < 0.05, Wilcoxon rank-sum test. (D) Heatmap of communication interaction weight per strength from CellChat analysis in control and IL-33 deletion groups. Left: M2 (ligand) to other leukocyte subsets as receivers. Right: M1 (ligand) to other leukocyte subsets as receivers. (E) Feature plot showing single cell expression of Tgfbr1 and Tgfbr2 in M1 or M2 subclusters. (F) Tgfb1 mRNA (left) or protein (right) expression in Treg cells stimulated with IL-33, IL-33+ST2, or saline. (G) Histogram and quantification of Arg-1 + -expressing F4/80 + macrophages that were co-cultured with Treg cells primed with or without IL-33. Control groups received IgG, and the neutralization group received a TGF-β1 antibody, all under M2-polarizing conditions (IL-4, 0.75 ng/mL). (H) Immunofluorescence images of F4/80 (red) and CD206 (green) in day 2 oral wounds of control and ΔIL33 Col1α2 mice. Scale bar, 500 μm. Inset: original wound edge; scale bar, 20 μm. (I and J) F4/80 + CD206 + M2 macrophage numbers normalized by stromal area (I) or by total F4/80 + macrophage numbers (J), comparing control and IL-33 deletion mice. (K) Total F4/80 + macrophage numbers between groups. Data represent mean ± SEM. n = 3–4 (F and G), where each N is an independent experiment from an individual control mouse; n = 5–7 mice (I–K). Student’s t test (I–K) or one-way ANOVA and post hoc test (F and G); * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Rat monoclonal anti-mouse F4/80 (clone CI:A3-1) , Bio-Rad , Cat. #: MCA497APC; RRID: AB_324435.

Techniques: Control, Single Cell, Expressing, Saline, Cell Culture, Neutralization, Immunofluorescence

Journal: Cell reports

Article Title: Fibroblast-derived alarmin promotes oral wound healing by activating regulatory T cells that relay pro-angiogenic and anti-inflammatory responses

doi: 10.1016/j.celrep.2025.116829

Figure Lengend Snippet: (A) H&E-stained images (left) and epithelial gap quantification (right) of day 2 oral wounds from Foxp3-DTR mice that received saline or diphtheria toxin (DT). Scale bar, 500 μm. (B) Left: COL3A1 staining images from day 4 oral wounds. Right: COL3A1 + area in control and Treg cell ablation mice. Scale bar, 500 μm. (C) LY6C + VEGFA + monocyte numbers normalized by stromal area in day 2 or 4 oral wounds. (D) F4/80 + CD206 + macrophage numbers normalized by stromal area in day 2 or 4 oral wounds. (E) MIF and TGF-β1 levels by ELISA from day 2 wound lysates, normalized by tissue weight. (F) CD31 + blood vessel numbers normalized by stromal area in day 2 or 4 oral wounds. (G and H) Flow cytometry plots (G) and quantification (H) of CD74 + LY6C + monocytes and F4/80 + CD206 + macrophages in day 4 oral wounds of Treg cell ablation mice that received vehicle (+saline) or recombinant MIF and TGF-β1 (+rMIF/TGF-β1, 5 ng/1 ng per wound). (I and J) CD31 + blood vessels (I) and COL3A1 + stromal area (J) in the wounds of Treg cell ablation mice that received saline or rMIF/TGF-β1. Data represent mean ± SEM. n = 5–10 mice each. Student’s t test; * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Rat monoclonal anti-mouse F4/80 (clone CI:A3-1) , Bio-Rad , Cat. #: MCA497APC; RRID: AB_324435.

Techniques: Staining, Saline, Control, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Recombinant